Multiplexed proximity ligation-based method uncovers immune checkpoint activation in the context of the tumor microenvironment
The study focuses on the interaction between PD1 and PD-L1 immune checkpoint proteins, which play a crucial role in T cell inhibition and tumor evasion from the immune system. The researchers employed a Naveni in situ-plex technology that allows simultaneous visualization of the PD1/PD-L1 interaction and other relevant biomarkers in human tumor tissues. By detecting PD1/PD-L1 interaction (using Naveni PD1/PD-L1 Atto647N), the method provides valuable insights into the activation of the immune checkpoint axis within the tumor. The inclusion of additional biomarkers helps differentiate between T cell inhibition as a part of the normal immune response or as a sign of tumor evasion. This novel multiplexed approach has promising applications in immune oncology and can assist in patient stratification for immunotherapy.